Intermediate
ChR2

Part:BBa_S03425:Design

Designed by: Ed Boyden   Group: Guests   (2006-02-05)


Channelrhodopsin-2, first 315 amino acids


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 163
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 163
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 248
    Illegal XhoI site found at 286
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 163
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 163
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

References

  • Nagel G, Szellas T, Huhn W, Kateriya S, Adeishvili N, Berthold P, Ollig D, Hegemann P, Bamberg E. Channelrhodopsin-2, a directly light-gated cation-selective membrane channel. Proc Natl Acad Sci U S A. 2003 Nov 25;100(24):13940-5.
  • Boyden, E. S., Zhang, F., Bamberg, E., Nagel, G., and Deisseroth, K. (2005) Millisecond-timescale, genetically-targeted optical control of neural activity. Nature Neuroscience 8(9):1263-1268.